Browsing by Author "Yildiz-Ozturk, Ece"

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Advanced Bioengineering Applications with Eco-Technology Approach for Sustainable Development
(SPRINGER INTERNATIONAL PUBLISHING AG, 2023) Ece Yildiz-Ozturk; Ruhan Askin Askin-Uzel; Askin-Uzel, Ruhan; Yildiz-Ozturk, Ece; V Koval; Y Kazancoglu; ES Lakatos
Problems such as excessive consumption habits depletion of natural resources and global climate change keep the concepts of both eco-entrepreneurship and bioeconomy on the agenda. Within the scope of the study agro-food-oriented bioengineering practices inwhich biotechnology is at the center were examined in the circular economy line. In the review eco-technological applications under field-specific titles have progressed in the form of examining academic and sectoral studies in the world. Hence it has been determined that the need for interdisciplinary improvement in biosystems has become an important issue on international scale especially in recent years. In this way it has been seen that significant contributions will be made in finding solutions to the productivity and efficiency problems that arise in eco-technological bioengineering applications especially with the contribution of entrepreneurship ecosystem studies. For this reason it is essential to carry out studies in which all actors involved in agriculture-food-biotechnology systems can come together. Therefore existing problem areas in bioengineering applications for sustainable development should be identified and joint solution proposals should be developed for the identified problem areas.
Green Approaches to Enhance Bioactive Compounds in Goji Berry (Lycium Barbarum) Fruits: Comparative Optimization of Pressurized Water, Microwave-Assisted, and Ultrasound-Assisted Extraction Technologies by Using Response Surface Methodology
(Wiley, 2025) Yildiz-Ozturk, Ece
Goji berries (Lycium barbarum L.), a superfruit with a long history of usage in Asian medicine, are gaining recognition for their potential as functional foods because of their high levels of antioxidants, flavonoids, anthocyanins, and phenolic acids. With the growing demand from consumers for clean-label and naturally sourced ingredients, environmentally friendly extraction technologies are now crucial to creating bioactive-rich extracts appropriate for food and nutraceutical applications. Three eco-friendly extraction methods-pressurized water extraction (PWE), microwave-assisted extraction (MAE), and ultrasound-assisted extraction (UAE)-are thoroughly evaluated in this study to maximize the bioactive compounds' recovery from Goji berry fruits. Water was the only solvent used in all extraction processes, guaranteeing environmental sustainability and food-grade compliance. The solid/liquid ratio, temperature, duration, pressure, and power were all optimized using response surface methodology (RSM). The total phenolic content (TPC), total flavonoid content (TFC), total anthocyanin content (TAC), and antioxidant activity (DPPH inhibition) of the extracted materials were assessed. Under ideal circumstances, the extracts' rutin contents were ascertained by HPLC analysis. According to the findings, MAE had the highest DPPH inhibition rate (75.942%), whereas PWE had the most TPC (17.753 mg GAE/g extract). The flavonoid content of both techniques was comparable. The UAE produced the best energy-to-bioactivity ratio and the most anthocyanin-rich extracts (3.607 mg C3G/g). UAE is the most ecologically friendly option among the techniques, as evidenced by its highest overall efficiency in terms of bioactive recovery and antioxidant capacity. This is the first study to employ a combined approach of RSM and bioactivity-energy efficiency assessment to optimize and compare water-based PWE, MAE, and UAE methods for Goji berries. These results demonstrate that green extraction technologies can be leveraged to sustainably produce bioactive compounds from functional foods like Goji berries, which have significant applications in food, nutraceuticals, and cosmetics.
Citation - WoS: 3
Citation - Scopus: 3
Novel Approach Methodologies in Modeling Complex Bioaerosol Exposure in Asthma and Allergic Rhinitis Under Climate Change
(CAMBRIDGE UNIV PRESS, 2025) Esra Atalay-Sahar; Ece Yildiz-Ozturk; Su Ozgur; Arzu Aral; Emre Dayanc; Tuncay Goksel; Ralph Meuwissen; Ozlem Yesil-Celiktas; Ozlem Goksel; Atalay-Sahar, Esra; Dayanc, Emre; Goksel, Tuncay; Aral, Arzu; Ozgur, Su; Yildiz-Ozturk, Ece; Goksel, Ozlem
The undeniable impact of climate change and air pollution on respiratory health has led to increasing cases of asthma allergic rhinitis and other chronic non-communicable immune-mediated upper and lower airway diseases. Natural bioaerosols such as pollen and fungi are essential atmospheric components undergoing significant structural and functional changes due to industrial pollution and atmospheric warming. Pollutants like particulate matter(PMx) polycyclic aromatic hydrocarbons(PAHs) nitrogen dioxide(NO2) sulfur dioxide(SO2) and carbon monoxide(CO) modify the surface and biological properties of atmospheric bioaerosols such as pollen and fungi enhancing their allergenic potentials. As a result sensitized individuals face heightened risks of asthma exacerbation and these alterations likely contribute to the rise in frequency and severity of allergic diseases. NAMs such as precision-cut lung slices(PCLS) air-liquid interface(ALI) cultures and lung-on-a-chip models along with the integration of data from these innovative models with computational models provide better insights into how environmental factors influence asthma and allergic diseases compared to traditional models. These systems simulate the interaction between pollutants and the respiratory system with higher precision helping to better understand the health implications of bioaerosol exposure. Additionally NAMs improve preclinical study outcomes by offering higher throughput reduced costs and greater reproducibility enhancing the translation of data into clinical applications. This review critically evaluates the potential of NAMs in researching airway diseases with a focus on allergy and asthma. It highlights their advantages in studying the increasingly complex structures of bioaerosols under conditions of environmental pollution and climate change while also addressing the existing gaps challenges and limitations of these models.
Citation - WoS: 2
Citation - Scopus: 2
Optimization of Green Extraction Techniques for Polyphenolics in Pinus brutia Bark Extract and Steam Gasification of the Remaining Fraction to Obtain Hydrogen-Rich Syngas and Activated Carbon
(American Chemical Society, 2024) Ece Yildiz-Ozturk; Pelin Secim-Karakaya; Fikret Müge Alptekin; Melih Soner Celiktas; Secim-Karakaya, Pelin; Celiktas, Melih Soner; Alptekin, Fikret Muge; Yildiz-Ozturk, Ece
Utilization of renewable resources has become imperative and considerable efforts have been devoted to tackling diverse global sustainability challenges which contribute to the circular economy. The focus of this work was to optimize the extraction of polyphenolic compounds in Pinus brutia bark using microwave-assisted (MAE) and ultrasonically assisted (UAE) extractions and evaluate the biological efficacies of the extracts. Additionally the residue of the extracted pine bark was subjected to steam gasification to produce hydrogen-rich syngas and activated carbon. The optimum process parameters for MAE were determined as 70 °C 10 min and 900 W and 987.32 mg gallic acid equivalent (GAE) 23.7 mg quercetin/g extract and 86.2% antioxidant activity were obtained. The optimum process parameters for UAE were determined as 70 °C 20 min and 50% power and 811.84 mg gallic acid equivalent (GAE) 30.1 mg quercetin/g extract and 90.8% antioxidant efficiency were obtained. The extracts obtained under optimized conditions were assessed for the bioactive phenolic compounds taxifolin (−)-catechin (−)-epicatechin and (−)-epicatechin gallate by ultra performance liquid chromatography (UPLC). Especially in MAE (ethanol) taxifolin content was notable (34.0 mg/g extract) followed by UAE (ethanol) (23.5 mg/g extract). Compared to MAE (ethanol) and UAE (ethanol) with regards to catechin content 1.05 mg/g extract and 0.81 mg/g extract were obtained respectively. Catalytic and noncatalytic steam gasification of pine bark residue yielded 57.3 and 60.8 mol % H2 respectively. In addition excellent tar reduction was achieved through utilizing a 10% boron-modified CaO alkali catalyst and the obtained activated carbon exhibited 1358.32 m2/g Brunauer-Emmett-Teller (BET) surface area and 1.05 cm3/g total pore volume which has potential use as an adsorbent for removing heavy metals and electrode material for supercapacitor application. © 2024 Elsevier B.V. All rights reserved.
Citation - WoS: 7
Citation - Scopus: 7
Organotypic lung tissue culture as a preclinical model to study host- influenza A viral infection: A case for repurposing of nafamostat mesylate
(Elsevier Ltd, 2024) Pelin Saglam-Metiner; Ece Yildiz-Ozturk; Aslı Tetik Vardarlı; Candan Çiçek; Ozlem Keskin Goksel; Tuncay Göksel; Beril Tezcanli; Ozlem Yesil-Celiktas; Yesil-Celiktas, Ozlem; Goksel, Tuncay; Saglam-Metiner, Pelin; Cicek, Candan; Yildiz-Ozturk, Ece; Tetik-Vardarli, Aslı; Goksel, Ozlem
Reliable and effective models for recapitulation of host-pathogen interactions are imperative for the discovery of potential therapeutics. Ex vivo models can fulfill these requirements as the multicellular native environment in the tissue is preserved and be utilized for toxicology vaccine infection and drug efficacy studies due to the presence of immune cells. Drug repurposing involves the identification of new applications for already approved drugs that are not related to the prime medical indication and emerged as a strategy to cope with slow pace of drug discovery due to high costs and necessary phases to reach the patients. Within the scope of the study broad-spectrum serine protease inhibitor nafamostat mesylate was repurposed to inhibit influenza A infection and evaluated by a translational ex vivo organotypic model in which human organ-level responses can be achieved in preclinical safety studies of potential antiviral agents along with in in vitro lung airway culture. The safe doses were determined as 10 µM for in vitro whereas 22 µM for ex vivo to be applied for evaluation of host-pathogen interactions which reduced virus infectivity increased cell/tissue viability and protected total protein content by reducing cell death with the inflammatory response. When the gene expression levels of specific pro-inflammatory anti-inflammatory and cell surface markers involved in antiviral responses were examined the significant inflammatory response represented by highly elevated mRNA gene expression levels of cytokines and chemokines combined with CDH5 downregulated by 5.1-fold supported the antiviral efficacy of NM and usability of ex vivo model as a preclinical infection model. © 2024 Elsevier B.V. All rights reserved.
Citation - Scopus: 3
Utilization of agri-food products as sources of high value-added bioactive compounds by using sustainable energy efficient eco-friendly technologies
(EDP Sciences, 2024) Ece Yildiz-Ozturk; Ruhan Askin-Uzel; Askin-Uzel, Ruhan; Yildiz-Ozturk, Ece; Y. Kazancoglu , S. Karamperidis , S. Jagtap , V. Koval
Due to the increasing population we continue to irresponsibly consume the important resources that we must leave as a legacy to future generations especially since the last century due to overconsumption and inadequate recovery. It has revealed the concept of sustainable development which essentially cares about people and envisages careful consumption of natural and cultural resources considering the needs of future generations in the process necessary to meet economic and social development. For healthy generations and a sustainable future we have to take measures to transform towards more sustainable agriculture-food systems protect natural resources adopt a more responsible production approach against the negative effects of climate change and prevent waste in all areas. There is a need to develop collaborations between different disciplines and sectors in order to support more efficient inclusive durable and sustainable agriculture-food systems and to approach the issue more holistically. Based on this point the applications of sustainable environmentally friendly and energy efficient modern advanced technologies in the food and agriculture sectors have been evaluated. More specifically the human health benefits of bioactive compounds in agri- food products approaches to the preservation and processing of these compounds in agri-foods and future perspectives in their sustainable- circular management are discussed. © 2024 Elsevier B.V. All rights reserved.