Melatonin mediated tolerance to benzalkonium chloride phytotoxicity through improved growth photochemical reactions and antioxidant system in wild-type and snat2 mutant Arabidopsis lines

Abstract

Melatonin (Mel) is a phytohormone that plays a crucial role in various plant processes including stress response. Despite numerous studies on the role of Mel in stress resistance its significance in plants exposed to benzalkonium chloride (BAC) pollution remains unexplored. BAC a common antiseptic poses a threat to terrestrial plants due to its widespread use and inefficient removal leading to elevated concentrations in the environment. This study investigated the impact of BAC (0.5 mg L−1) pollution on wild-type Col-0 and snat2 knockout mutant Arabidopsis lines revealing reduced growth altered water relations and gas exchange parameters. On the other hand exogenous Mel (100 μM) treatments mitigated BAC-induced phytotoxicity and increased the growth rate by 1.8-fold in Col-0 and 2-fold in snat2 plants. snat2 mutant seedlings had a suppressed carbon assimilation rate (A) under normal conditions but BAC contamination led to further A repression by 71% and 48% in Col-0 and snat2 leaves respectively. However Mel treatment on stressed plants was successful in improving F<inf>v</inf>/F<inf>m</inf> and increased the total photosynthesis efficiency by regulating photochemical reactions. Excessive H<inf>2</inf>O<inf>2</inf> accumulation in the guard cells of plants exposed to BAC pollution was detected by confocal microscopy. Mel treatments triggered almost all antioxidant enzyme activities (except POX) in both Arabidopsis lines under stress. This enhanced antioxidant activity facilitated by foliar Mel application contributed to the alleviation of oxidative damage regulation of photosynthesis reactions and promotion of plant growth in Arabidopsis. In addition to corroborating results observed in many agricultural plants regarding the development of tolerance to environmental stresses this study provides novel insights into the action mechanisms of Mel under the emerging pollutant benzalkonium chloride. © 2024 Elsevier B.V. All rights reserved.