Dinamik Mikroakışkan Hücre Kültürü Platformlarında Difüzyon-Konveksiyon Proseslerinin Hesaplamalı Akışkanlar Dinamiği Modellemesi
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Date
2022
Authors
Ece Yildiz-Ozturk
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Abstract
Mikroakışkan tabanlı biyokimyasal analizler hücre ve doku mühendisliğindeki son gelişmeler mikroakışkan sistemler içinde uzaysal-zamansal olarak kütle transferinin kontrol edilmesine dayanmaktadır. Mikroakışkan sistemler ile hücresel mikroçevrenin kontrol edilmesi ve doku benzeri yapıların taklit edilmesinin yanısıra yüksek verimli analizlerin gerçekleştirilmesinde de oldukça kullanışlı araçlardır. Bu sistemler biyoaktif moleküllerin nütrientlerin büyüme faktörlerinin ve diğer hücresel regülatör moleküllerin konsantrasyon gradientlerinin zamana ve konuma bağlı olarak kontrol etme potansiyeline sahiptir. Bu nedenle mikrosistemlerde biyomoleküllerin taşınım olaylarının modellenmesi mikroakışkan platformların tasarımını ve kantitatif biyolojik analizleri kolaylaştıran değerli ve kullanışlı bir analitik araçtır. Geliştirilen analitik model mikroakışkan sistem tasarımına rehberlik ettiğinden, maliyetli ve zaman alıcı deneyler minimuma indirilebilmekte, tasarım süreci verimliliği ve etkinliği arttırılabilmektedir. Çalışma kapsamında ilaç taşınım uygulamalarına yönelik tek kanallı mikroakışkan platformda biyomoleküllerin difüzyon ve konveksiyon proseslerine vurgu yaparak kütle transferi profilini gösteren analitik bir model oluşturulmuştur. Mikroakışkan hücre kültürü sistemlerinin hücresel fizyolojik ortamların akış dinamiğini taklit edebilmesi için pulsatil laminar sıvı akışını sağlayabilen peristaltik pompa ile sistem kurulmuştur. Dinamik akış koşulları altında hücreler üzerine etki eden biyomekanik kuvvetlerin (akış hızı konsantrasyon basınç dağılımı ve kayma gerilimi) etkisini sayısal olarak incelemek için COMSOL Multiphysics sonlu elemanlar yazılımı kullanılarak mikroakışkan sistemin sayısal simülasyonu yapılmıştır. Mikroakışkan sistemde hücreler minimum kayma gerilimine maruz bırakılırken kanal uzunluğu boyunca konsantrasyon profilinin korunduğu gösterilmiştir. Akış hızının arttırılmasıyla mikro kanal boyunca çözünen konsantrasyon dağılımı değiştirilebilmektedir ve bu da hücreler üzerindeki kayma gerilimini arttırmaktadır. Simülasyonu yapılan mikroakışkan analitik modelin hücre kültürü biyolojik analizler ve ilaç taşıyıcı sistemler için kullanılacak mikroakışkan platformların geliştirilmesinde sistem tasarımı ve parametre seçimi için temel olarak kullanılabileceği düşünülmüştür.
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ORCID
Keywords
Mikroskopi-Biyoloji-Mühendislik- Biyotıp-Tıbbi Araştırmalar Deneysel-Kimya- Uygulamalı, Tıbbi Araştırmalar Deneysel, Kimya, Uygulamalı, Biyoloji, Mühendislik, Biyotıp, Mikroskopi
Fields of Science
0301 basic medicine, 0303 health sciences, 03 medical and health sciences
Citation
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